EVALUATION OF MICROBIOLOGICAL QUALITY OF TANK WATER AT THE INSTITUTE OF MANAGEMENT AND TECHNOLOGY {IMT} CAMPUS III ENUGU

EVALUATION OF MICROBIOLOGICAL QUALITY OF TANK WATER AT THE INSTITUTE OF MANAGEMENT AND TECHNOLOGY {IMT} CAMPUS III ENUGU

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ABSTRACT

The evaluation of microbiological quality of tank water at the Institute of Management and Technology campus III Enugu was carried out by using ten samples of water obtained from ten different tank waters. For viable plate count for the presence of pathogenic organisms and organisms indicating organic matter pollution, plating technique of pour plates were used, also presence of coliform groups and Escherichia  coli were examined using the Presumptive, Confirmatory and Completed  coliform test techniques. The media used include Nutrient agar, Brilliant green bile agar, Eosin Methylene Blue agar (EMB), MacConkey broth and Brilliant green bile broth. The total viable plate count gave 31 X 10-3 cfu/ml for pathogenic organisms and 21 X 10-3 cfu/ml for organisms indicating organic matter pollution, whereas the presumptive test showed the presence of coliform groups in seven (7) samples of water out of ten. The confirmatory test showed the presence of colform in five (5) samples of water whereas the presence of Escherichia coli was shown in three (3) samples. The completed test result revealed a highest coliform count of 2 cfu in one sample of water while 1cfu was obtained in three (3) water samples for Escherichia coli. The result indicated the presence of coliform and Escherichia coli but from the deduction, the microbial load was minimal in all the tests. Therefore, the tank water at campus III of IMT is not fit for drinking though it can be used for other domestic purposes.

TABLE OF CONTENTS
Title page                                    i
Certification                                    ii
Dedication                                    iii
Acknowledgement                                    iv
Table of contents                                v
List of Tables                                    viii
Abstract                                    ix

CHAPTER ONE    INTRODUCTION   
Background of Study                            1  
Statement of  the Problem                         2   
Objective of the Study                            3   
Hypothesis                                3   
Significance of the study                        3  
Limitations of the study                        4

CHAPTER TWO    LITERATURE REVIEW                            5  
Sources of Tank Water                                   6   
Drinking Water                                8   
Drinking Water Standard                                8   
Tank water Contamination                        10   
Tank Water purification/Treatment                        12  
Water pollution Indicator                        15

CHAPTER THREE    MATERIALS AND METHODS           
Materials (see appendix)                        18   
Methods                                    18    3.2.1    
Sterilization of Materials                    18     
Collections of Water Samples                18     
Preparation of Culture Media                19    
Dilution of Water Samples                    19   
Examination of Water Samples                    
Total Viable Plate Counts                    20    
Bacteriological Examination                21

CHAPTER FOUR    RESULT                                        25   
Total Viable Plate Counts                        25   
Coliform Test                                
Presumptive  Test                        26
 Confirmatory Test                        27
Completed Test                            29

CHAPTER FIVE DISCUSSION, CONCLUSION AND RECOMMENDATION     Discussion                                    30    
Conclusion                                    33    
Recommendation                            34
REFERENCES                                        35
APPENDIX                                            37

LIST OF TABLES
Table 1:Total Viable Plates Count for pathogenic organisms and organisms for organic matter pollution present in the ten samples of water     25
Table 2: Presumptive test for the number of tubes positive for coliform organisms                                            26
Table 3: Confirmatory test for coliform organisms and Escherichia   coli present in the water samples and their most probable number (mpn).     27
Table 4: Completed test for coliform organisms and Escherichia coli.    29

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