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The main aim of this research was to characterize the enterobacterial uropathogens with respect to drug resistance in Awka, south-eastern Nigeria. The following enterobacterial uropathogens isolated from patients attending Chukwuemeka Odumegwu Ojukwu University Teaching Hospital, Awka were studied: E.coli (58), Salmonella spp. (15), K. pneumoniae (14), Citrobacter freundii (10) and Enterobacter aerogenes(3). Their antibiotic susceptibility patterns against major classes of antibiotics were evaluated using agar disk diffusion assay. The resistant uropathogens were analyzed for the presence of biofilm using modified Christensen method and the presences of beta-lactamases among the isolates were checked using multiplex PCR. The antibiotic susceptibility of the isolates showed that 60% of the E.coli isolates were resistant to cefpodoxime but were highly susceptible to the fluoroquinolones with the exception of ciprofloxacin. Salmonella isolates on the other hand, had a very good susceptibility profile to the 3rd generation cephalosporins but were resistant to ofloxacin and cotrimoxazole. Klebsiella pneumoniae isolates were resistant to cefpodoxime, cefotaxime and cotrimoxazole but sensitive to the fluoroquinolones. Biofilm producing isolates were 74% while the non biofilm formers were 26%. Out of the 54 E. coli that were screened for ESBL production, 35 were confirmed positive for ESBL production with PCR. The predominant ESBL gene was blaTEM with blaSPM being the most predominant MBL gene. Out of the 21 isolates that were screened positive for AmpC production, only one was confirmed positive using PCR. Nine out of 10 K.pneumoniae that was screened to be positive for ESBL production were confirmed positive by PCR. All the 10 C. freundii were positive for ESBL genes and some organisms had co-expression of genes. This study showed high prevalence of drug resistant genes among the enterobacterial uropathogens.
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SIMILAR INDUSTRIAL CHEMISTRY FINAL YEAR PROJECT RESEARCH TOPICS
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1. PRODUCTION OF MOSQUITOES REPELLANTS INSECTICIDES (MOSQUITO COIL)
» CHAPTER ONE INTRODUCTION 1.0 INSECTICIDES (General Overview) Insecticide is a substance or a mixture of substances used for killing insects[1]. It is ...Continue Reading »Item Type & Format: Project Material - Ms Word | 52 pages | Instant Download | Chapter 1-5 | INDUSTRIAL CHEMISTRY DEPARTMENT
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2. CHEMISTRY AND THERAPEUTIC EFFECTS OF ANALGESICS
» CHAPTER 1 INTRODUCTION This work, Chemistry and Therapeutic Effects of Analgesics is an effort to bring together various works which has been done abo...Continue Reading »Item Type & Format: Project Material - Ms Word | 52 pages | Instant Download | Chapter 1-5 | INDUSTRIAL CHEMISTRY DEPARTMENT
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3. ASSESSMENT OF SOME HEAVY METALS IN SOIL SAMPLES ALONG GUTTERS IN NKPOR, ANAMBRA STATE
» ABSTRACT Ten representative soil samples were collected along major gutters within Nkpor metropolis. The soil samples were digested using mixtures of ...Continue Reading »Item Type & Format: Project Material - Ms Word | 57 pages | Instant Download | Chapter 1-5 | INDUSTRIAL CHEMISTRY DEPARTMENT
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4. ASSESSING THE EFFECT OF DRY PLANTAIN AND BANANA PEELS ON MUD RHEOLOGICAL PROPERTIES
» TABLE OF CONTENTS Contact Page Title page i Declaration ii Certification iii Dedication iv Acknowledgement v Table of Contents vi List of Tables viii ...Continue Reading »Item Type & Format: Project Material - Ms Word | 33 pages | Instant Download | Chapter 1-5 | INDUSTRIAL CHEMISTRY DEPARTMENT
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5. THE BLEACHING OF PALM OIL USING ACTIVATED CHARCOAL
» ABSTRACT This project work is on the bleaching of palm oil using activated charcoal. The charcoal used for this work was made from pieces of animal bo...Continue Reading »Item Type & Format: Project Material - Ms Word | 80 pages | Instant Download | Chapter 1-5 | INDUSTRIAL CHEMISTRY DEPARTMENT
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6. THE PRODUCTION OF BIODIESEL (FAME) FROM PALM KERNEL OIL (PKO) USING CONCENTRATED SULPHURIC ACID AS CATALYST
» CHAPTER ONE INTRODUCTION 1.0 GENERAL INTRODUCTION Energy is a fundamental pillar of modern society as well as being an essential building block for so...Continue Reading »Item Type & Format: Project Material - Ms Word | 45 pages | Instant Download | Chapter 1-5 | INDUSTRIAL CHEMISTRY DEPARTMENT
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7. COMPARATIVE STUDY BETWEEN OVERBALANCED AND UNDERBALANCED OIL DRILLING METHODS
» ABSTRACT The technique used worldwide while drilling is known as overbalanced drilling. Which is defined as the drilling process where the hydrostatic...Continue Reading »Item Type & Format: Project Material - Ms Word | 52 pages | Instant Download | Chapter 1-5 | INDUSTRIAL CHEMISTRY DEPARTMENT
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8. INSECTICIDAL POTENTIAL OF Hyptis Suaveolens USING DIFFERENT EXTRACTS (ETHANOL, N-HEXANE AND CHLOROFORM).
» CHAPTER ONE INTRODUCTION 1.1 Background of the Study The development of organic chemistry took place along with the study of plants, mainly in the 19t...Continue Reading »Item Type & Format: Project Material - Ms Word | 36 pages | Instant Download | Chapter 1-5 | INDUSTRIAL CHEMISTRY DEPARTMENT
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9. THE INHIBITORY EFFECT OF ETHANOLIC EXTRACT OF MORINGA OLEIFERA LEAF ON THE CORROSION OF MILD STEEL IN 5 M AND 1 M HYDROCHLORIC ACID (HCl)
» CHAPTER ONE INTRODUCTION 1.1 Background of study Corrosion of materials has continued to receive interest in the technological world as its effects on...Continue Reading »Item Type & Format: Project Material - Ms Word | 60 pages | Instant Download | Chapter 1-5 | INDUSTRIAL CHEMISTRY DEPARTMENT
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10. ENHANCING THE PROPERTIES OF COAL BRIQUETTE USING SPEAR GRASS (IMPERATA CYLINDRICA) AND ELEPHANT GRASS (PENNISETUM PURPUREUM)
» ABSTRACT The urgent need to protect our forest, to mitigate health hazards faced by the people from the use of firewood for cooking and to find an eff...Continue Reading »Item Type & Format: Project Material - Ms Word | 100 pages | Instant Download | Chapter 1-5 | INDUSTRIAL CHEMISTRY DEPARTMENT