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Crude oil and kerosene have useful applications in the society and are, therefore, used as sources of income and energy. However, the spills arising from crude oil and kerosene in our environment are becoming a visible problem in our ecosystem. Crude oil and kerosene pollute our farmlands and other arable lands and may negatively affect the growth and activities of nitrifying bacteria responsible for nitrification process, leading to loss of soil fertility. Considering the important roles played by nitrifying bacteria in soil fertility in our ecosystem, the assessment of the response of the nitrifying bacteria to pollution stress exerted by crude oil and kerosene on them was investigated. Two genera of nitrifying bacteria were isolated from the soil and were identified asNitrosomonas sp and Nitrobacter sp based on their morphological, biochemical and physiological characteristics. Nitrosomonas sp was isolated using Winogradsky medium for nitrification phase I while Nitrobacter sp was isolated using Winogradsky medium for nitrification phase II. A range of crude oil and kerosene concentrations (in triplicates) were prepared and introduced into sterile Winogradsky salt broth to arrive at final concentrations of 0.5%, 1%, 2%, 5% and 10% (v/v) respectively. The flasks were thereafter inoculated with standardized pure cultures of Nitrosomonas sp and Nitrobacter sp using a uniform inoculum size of 1ml of 1.5 x106 cells for Nitrosomonas sp and 1.2 x106 cells for Nitrobacter sp and incubated for 120 hours. The result of the toxicity test carried out on these isolates showed that the sensitivity of each test organism was a function of both the contact time and concentrations of crude oil and kerosene. The effect of different concentrations of crude oil and kerosene on the oxidation of nitrite to nitrate showed that oxidation of nitrite to nitrate was hindered at higher concentrations of the two pollutants, but, at lower concentrations, crude oil and kerosene did not hinder oxidation of nitrite to nitrate. Information generated from various tests can be of use in the management of pollution for the purpose of prediction of environmental effects of crude oil and kerosene, comparison of toxicants or organisms or test conditions and regulation of crude oil and kerosene spills. For instance this study will help us to provide information for regulatory purposes that a soil contaminated with certain percentage of crude oil or kerosene should not be used for agricultural purposes or practices.
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SIMILAR MICROBIOLOGY FINAL YEAR PROJECT RESEARCH TOPICS
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1. EFFECT OF MORINGA OLEIFERA LEAF EXTRACT ON THE GROWTH AND YIELD OF PEPPER
» ABSTRACT This study was carried out during the two summer seasons of 2014 and 2015 on pepper (Capsicum annuum L.) cv. California Wonder. The goals of ...Continue Reading »Item Type & Format: Project Material - Ms Word | 31 pages | Instant Download | Chapter 1-5 | MICROBIOLOGY DEPARTMENT
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2. ANTIBACTERIAL ACTIVITIES OF Carica papaya SEED AGAINST ENTERIC ORGANISMS
» ABSTRACT Lots of research has been conducted on Carica papaya and its antibacterial efficacy on bacterial pathogens and this led to this study on anti...Continue Reading »Item Type & Format: Project Material - Ms Word | 37 pages | Instant Download | Chapter 1-5 | MICROBIOLOGY DEPARTMENT
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3. EVALUATION OF THE PREVENTIVE EFFECT OF DIETARY INCLUSION OF HYPHAENE THEBAICA FRUIT (LINN) ON N-METHYL-N-NITROSOUREA-INDUCED COLON CARCINOGENESIS IN M...
» ABSTRACT The preventive effect of dietary inclusion of Hyphaene thebaica fruit at 0%, 2.5%, 5% and 10% on colon carcinogenesis initiated with N methyl...Continue Reading »Item Type & Format: Project Material - Ms Word | 52 pages | Instant Download | Chapter 1-5 | MICROBIOLOGY DEPARTMENT
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4. ISOLATION, IDENTIFICATION AND ANTIBIOGRAM OF STAPHYLOCOCCUS AUREUS ISOLATED FROM COW MEAT
» AbstractThe study was carried out with aim to isolate Staphylococcus aureus from cow meat and determine the antibiogram pattern of S. aureus. Three sa...Continue Reading »Item Type & Format: Project Material - Ms Word | 52 pages | Instant Download | Chapter 1-5 | MICROBIOLOGY DEPARTMENT
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5. HEPATITIS C VIRUS AMONG PREGNANT WOMENPEOPLE LIVING WITH HIVAIDS ATTENDING CLINIC AT UNTH ITUKUOZALLA
» ABSTRACT A total of 50 blood (30 pregnant and 20 HIV) samples were obtained from pregnant women and PLWHA attending clinic at UNTH Ituku ozalla. Sampl...Continue Reading »Item Type & Format: Project Material - Ms Word | 39 pages | Instant Download | Chapter 1-5 | MICROBIOLOGY DEPARTMENT
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6. ISOLATION AND IDENTIFICATION OF MICROORGANISM IN STREAM WATER [ATOLAGBE] IREE TOWN
» ABSTRACT The stream water was examined for microorganism present in it. The isolated and identified bacteria are Bacillus cereus and Staphylococcus au...Continue Reading »Item Type & Format: Project Material - Ms Word | 52 pages | Instant Download | Chapter 1-5 | MICROBIOLOGY DEPARTMENT
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7. BACTERIOLOGICAL AND PHYSICOCHEMICAL ANALYSIS OF PUBLIC DRINKING WATER SOURCES IN SOKOTO METROPOLIS
» ABSTRACT Bacteriological and physicochemical properties of drinking water (tap, well and borehole) in Sokoto metropolis were investigated to determine...Continue Reading »Item Type & Format: Project Material - Ms Word | 61 pages | Instant Download | Chapter 1-5 | MICROBIOLOGY DEPARTMENT
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8. SUSCEPTIBILITIES OF Salmonella typhi AND OTHER BACTERIAL PATHOGENS TO ANTIBIOTICS AND HOT AQUEOUS EXTRACT OF Hibiscus sabdariffa
» ABSTRACT The susceptibilities of Salmonella typhi and other pathogens to antibiotics and hot aqueous extract of Hibiscus sabdariffa were investigated ...Continue Reading »Item Type & Format: Project Material - Ms Word | 110 pages | Instant Download | Chapter 1-5 | MICROBIOLOGY DEPARTMENT
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9. PREVALENCE OF MULTIDRUG RESISTANT LIVESTOCK ASSOCIATED STAPHYLOCOCCUS AUREUS ISOLATED FROM NASAL PASSAGE OF HEALTHY CATTLE IN KARA MARKET OGUN STATE N...
» ABSTRACT Staphylococcus aureus, ‘one of the most adaptable and virulent pathogens in modern times’ is a facultative anaerobic Gram positiv...Continue Reading »Item Type & Format: Project Material - Ms Word | 82 pages | Instant Download | Chapter 1-5 | MICROBIOLOGY DEPARTMENT
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10. PREVALENCE AND ANTIMICROBIAL SUSCEPTIBILITY OF GRAM-NEGATIVE BACTERIA IN URINE
» CHAPTER ONE INTRODUCTION 1.1 Background. Urinary tract infection (UTI) is defined as the bacterial invasion of the urinary tract. It is primarily caus...Continue Reading »Item Type & Format: Project Material - Ms Word | 77 pages | Instant Download | Chapter 1-5 | MICROBIOLOGY DEPARTMENT